Analysis of Growth Factor Signaling in Embryos (Methods in by Malcolm Whitman, Amy Sater

By Malcolm Whitman, Amy Sater

Developmental biologists were pushed to enquire development issue signaling in embryos so as to comprehend the regulatory mechanisms underlying a given developmental procedure. therefore, it really is severe to discover the technical tools and experimental designs for progress issue signaling in embryos.

Focusing on particular pathways or pathway parts, Analysis of progress issue Signaling in Embryos presents the equipment and instructions for experimental layout to review significant elements of phone signaling in vertebrate embryos. The e-book covers a huge diversity of subject matters in signaling and quite a few present version organisms. part I explores particular signaling pathways or pathway elements. during this part, a few chapters spotlight the biochemistry of signaling pathways in the course of improvement, that is frequently targeted from that saw in cellphone tradition structures. part II discusses ionic regulatory mechanisms and the 2 chapters in part III research methods of investigating gene rules according to extracellular signs. eventually, part IV addresses rising suggestions that facilitate built-in analyses of mobile signaling in vivo in embryonic platforms.

Featuring contributions from professional researchers, Analysis of progress issue Signaling in Embryos will offer a beginning for extra explorations of the mobile regulatory mechanisms governing vertebrate embryonic development.

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Lee, J. , Casein kinase I phosphorylates the Armadillo protein and induces its degradation in Drosophila, Embo J 21 (7), 1733–42, 2002. 55. , Identification of a domain of Axin that binds to the serine/threonine protein phosphatase 2A and a self-binding domain, J Biol Chem 274 (6), 3439–45, 1999. 56. , Domains of Axin involved in protein-protein interactions, Wnt pathway inhibition, and intracellular localization, J Cell Biol 145 (4), 741–56, 1999. 57. , Logan, C. , A Drosophila Axin homolog, Daxin, inhibits Wnt signaling, Development 126 (18), 4165–73, 1999.

III. CONCLUSIONS Xenopus embryos offer a powerful tool for investigation of the canonical Wnt pathway. A wealth of assays are available to find new genes involved, to examine known proteins for unknown roles, and to gain detailed insight into the molecular mechanisms that underlie both the on and off states of Wnt signal transduction. It is important to use an integrated approach to evaluating the role of a particular protein in the Wnt pathway. Phenotypic assays should be supported by evidence of Wnt transcriptional effects and regulation of β-catenin stability.

15. , 17, 295, 2003. 16. , Wnt/Frizzled activation of Rho regulates vertebrate gastrulation and requires a novel Formin homology proteins Daam1, Cell, 107, 843, 2001. 17. C. , Dishevelled activates Ca2+ flux, PKC, and CamKII in vertebrate embryos, J Cell Biol, 161, 769, 2003. 18. Liu, T. , G protein signaling from activated rat frizzled-1 to the beta-cateninLef-Tcf pathway, Science, 292, 1718, 2001. 19. Liu, X. , Activation of a frizzled-2/beta-adrenergic receptor chimera promotes Wnt signaling and differentiation of mouse F9 teratocarcinoma cells via Galphao and Galphat, Proc Natl Acad Sci USA, 96, 14383, 1999.

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